1. Antibodies
  2. Homo sapiens
  3. FOXM1

ENCAB732JFX

Antibody against Homo sapiens FOXM1

Homo sapiens
K562, HEK293T
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
MCF-7
not characterized to standards
Status
released
Source (vendor)
GeneTex
Product ID
GTX102170
Lot ID
40373
Characterized targets
FOXM1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1211
External resources

Characterizations

FOXM1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody GTX102170. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 84.283
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOXM1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody GTX102170. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 84.283
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOXM1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody GTX102170. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 84.283
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOXM1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody GTX102170. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 84.283
Reviewer comment
the arrow is misleading, it seems that the higher band is the correct band
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 expt1042_2.jpg
FOXM1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody GTX102170. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 84.283
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOXM1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody GTX102170. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 84.283.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
FOXM1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody GTX102170, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
SFPQ, NUP205, ZMYM4, KPNA7, YIF1A, SART3, MDN1, DVL2, RIF1, NUP160, NUP93 and ALB are not DNA binding TFs.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford