1. Antibodies
  2. Homo sapiens
  3. MLLT1

ENCAB649WEQ

Antibody against Homo sapiens MLLT1

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A302-268A
Lot ID
1
Characterized targets
MLLT1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-799
External resources

Characterizations

MLLT1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A302-268A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
+20% tolerance would allow a 75.6kD band
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
MLLT1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A302-268A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
MLLT1 is component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), http://www.genecards.org/cgi-bin/carddisp.pl?gene=MLLT1&keywords=mllt1. Biogrid 9 publications about the interationof MLLT1 and AFF4, http://thebiogrid.org/110444/summary/homo-sapiens/mllt1.html
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
MLLT1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A302-268A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 62.056.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford