1. Antibodies
  2. Homo sapiens
  3. ATF3

ENCAB548QXS

Antibody against Homo sapiens ATF3

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Active Motif
Product ID
2/61715
Lot ID
unknown
Characterized targets
ATF3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
michael-snyder:AS-897

Characterizations

ATF3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody 2/61715. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 20.576.
Submitter comment
We attached two images, because we want to show that the target size band only showed in IP but not in IgG sample.
Reviewer comment
There is a faint but perceptible band.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ATF3 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target ATF3 is represented by the attached position weight matrix (PWM), and is derived from ENCFF958KNK. Motif enrichment analysis was done by Dr. Zhizhuo Zhang and Dr. Lei Hou (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.700347453 Global enrichment Z-score: 8.984221658 Positional bias Z-score: 8.118391146 Peak rank bias Z-score: 7.91734046 Enrichment rank: 1.0
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford