ENCAB521XDP
Antibody against Homo sapiens TCF12
Homo sapiens
K562, HepG2
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A300-755A
- Lot ID
- 1
- Characterized targets
- TCF12 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-1446
- External resources
Characterizations
TCF12 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A300-755A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 72.965
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1077_06_TCF12_A300-755A.jpg
TCF12 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A300-755A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 72.965.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- TCF12_A300-755A.jpg
TCF12 (Homo sapiens)
exempt from standards
- Caption
- IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A300-755A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
- Submitter comment
- All of ILF3, SMARCA5, NOP56, FUS, NOP58, EFTUD2 and NAT10were detected in MS of TCF12 (ENCAB506UYG).
- Reviewer comment
- TCF12 is not the highest ranked TF in the IP-MS analysis but SMARCA5, which is ranked higher is also detected in an independent IP-MS analysis of another TCF12 antibody (ENCAB506UYG) so it's possible that is due to a novel interaction not yet described in the literature. This case is permitted by the standards document.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- TCF12_A300-755A_final.pdf
TCF12 (Homo sapiens)
HepG2
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody A300-755A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 72.965.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- TCF12_A300-755A_HepG2.jpg