1. Antibodies
  2. Homo sapiens
  3. TBX3

ENCAB402UFE

Antibody against Homo sapiens TBX3

Homo sapiens
HepG2
characterized to standards with exemption
Status
released
Source (vendor)
Sigma
Product ID
HPA005799
Lot ID
A78565
Characterized targets
TBX3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
T-box transcription factor TBX3 recombinant protein epitope signature tag (PrEST)
Antigen sequence
QSMRVFDERHKKENGTSDESSSEQAAFNCFAQASSPAASTVGTSNLKDLCPSEGESDAEAESKEEHGPEACDAAKISTTTSEEPCRDKGSPAVKAHLFAAERPRDSGRLDKASPDSRHSPATISSSTR
Aliases
michael-snyder:782
External resources

Characterizations

TBX3 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody HPA005799. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
No major band indicated at >50% of the total signal in IP lane. Multiple bands could be acceptable with a knockdown rescue characterization.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
TBX3 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line HepG2 using the antibody HPA005799. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 79.389.
Submitter comment
We analyzed multiple immunoreactive bands by mass-spec.
Reviewer comment
Multiple bands but mass spec analysis showed the target TF to be highest ranked and not in IgG with no other site specific TFs above the target.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
TBX3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
exempt from standards
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from HepG2 nuclear cell lysates using the antibody HPA005799, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
SH3BP1 involves GTPase activator activity and SH3 domain binding. Both ACIN1 and TBX3 have interactions with ELAVL1 (36KD), http://thebiogrid.org/112788/summary/homo-sapiens/tbx3.html and http://thebiogrid.org/116634/summary/homo-sapiens/acin1.html, ELAVL1 is not in the same gel slice with ACIN1 and TBX3. HNRNPUL1 and HSPD1 were also detected in MS of TBX3 (ENCAB320XLX). KCTD3 has no transcription related function.
Reviewer comment
PF: Target is not in control IgG and no site-specific TFs above the target TF on the not in control IP list.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford