ENCAB350TQZ
Antibody against Homo sapiens EZH2
Homo sapiens
HepG2, K562, H1
characterized to standards
Homo sapiens
HCT116, SK-N-MC, PC-3, MM.1S
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 5246BF
- Lot ID
- 2
- Characterized targets
- EZH2 (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Antigen description
- enhancer of zeste homolog 2 (Drosophila)
- Aliases
- bradley-bernstein:PchAb 1135
- External resources
Characterizations
EZH2 (Homo sapiens)
HCT116SK-N-MCPC-3MM.1S
exempt from standards
- Caption
- This is a request for "exemption from further primary validation" for an additional EZH2 antibody. It was successfully western blotted in 3 cell types, and a secondary validation was accepted. We are asking for this antibody to be valid for a total of four additional cell types not included in the western blot we supplied to DCC.
- Submitter comment
- This antibody has been requested for exemption.
- Reviewer comment
- Peggy Farnham approved this request on behalf of the antibody standards working group.
- Submitted by
- Charles Epstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- exempted.png
EZH2 (Homo sapiens)
HepG2K562H1
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell lines H1-hESC (lane 2), HepG2 (lane 3), and K562 (lane 4) using the antibody ab6721 (Lot GR119224-3). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using rabbit IgG. Target molecular weight antibody: 98kDa. https://www.cellsignal.com/products/primary-antibodies/ezh2-d2c9-xp-rabbit-mab/5246
- Submitted by
- Kathrina Onate
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
EZH2 (Homo sapiens)
compliant
- Caption
- This validation relies on the use of antibodies to a chromatin regulator and a functionally related histone modification in MM.1S cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The first track shown used an antibody to phosphorylated EZH2 (PchAb 1135, ENCAB350TQZ), and the second track shown used an antibody to H3K27me3 previously characterized to Encode standards (PchAb 683, ENCAB000BLA). Overall genomewide correlation: >0.85
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- EZH2 PchAb 1135 WIP SAV.pdf