ENCAB301QZF
Antibody against Homo sapiens ZBTB5
Homo sapiens
K562
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A304-063A
- Lot ID
- 1
- Characterized targets
- ZBTB5 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-1402
- External resources
Characterizations
ZBTB5 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A304-063A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 74.278
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1079_12_ZBTB5_A303-063A.jpg
ZBTB5 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A304-063A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 74.278.
- Reviewer comment
- There are multiple immunoreactive bands but the marked band has >= 50% of the total signal in the lane as required by the standards.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- MS1027_6_ZBTB5-A304-063A.JPG
ZBTB5 (Homo sapiens)
exempt from standards
- Caption
- IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A304-063A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
- Submitter comment
- None of the proteins ranked above or equally ranked have been shown to be sequence-specific TFs (including ILF3, TR150, FUS, PNN, ADAR, TAF15, DDX1, TOP1).
- Reviewer comment
- Not all immunoreactive bands were analyzed by mass-spec. Obviously, it's too late fix that now and only what was submitted can be evaluated. The targeted TF can be detected
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ZBTB5_(A304-063A)_final.xls.pdf