ENCAB233VZE

Homo sapiens

any cell type or tissue

characterized to standards

K562

Method: immunoblot

compliant

Caption: We performed a WB using nuclear extracts from K562 (15ug) cells . Samples were resolved by electrophoresis on a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using an Invitrogen iBlot system (P3 8')' Membrane was blocked in TBST w/ 5% BSA for 1 hr @ RT. then blotted with primary antibody at the appropriate concentration in blocking solution O/N @ 4°C. Membrane was washed and blotted with secondary HRP-conjugated antibody appropriately diluted in blocking buffer. Detection was made using an ECL Detection Kit and incubated for 1 min. Bands of expected size (~17kDa) were detected using a ChemiDoc camera and visualized in extracts but not in lane with unmodified H3 Histone peptide. Antibody seems to be specific since no reactivity with non histone proteins or unmodified histones was detected.
Submitted by: Nina Farrell
Lab: Bradley Bernstein, Broad
Grant: UM1HG009390
Download: H3K36me3_ Cell Signaling _ Part 4909 _ Lot 5 _ WB _PchAb 1419.pdf

Method: dot blot assay

compliant

Caption: We performed a DB analysis using the large set of modified peptides provided in the Active Motif MODified Histone Peptide Array, catalog #13005. The only peptide recognized by the H3K36me3 antibody was the correct one.
Submitted by: Nina Farrell
Lab: Bradley Bernstein, Broad
Grant: UM1HG009390
Download: H3K36me3_Cell Signaling_4909_5_DB_ PchAb 1419.pdf