ENCAB189GDE
Antibody against Homo sapiens AGO1
Homo sapiens
K562, HepG2
characterized to standards
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 5053
- Lot ID
- 1
- Characterized targets
- AGO1 (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Isotype
- IgG
- Antigen description
- A synthetic peptide corresponding to the sequence around Ile392 of human Argonaute 1 protein
- Aliases
- xiang-dong-fu:EIF2C1
- External resources
Characterizations
AGO1 (Homo sapiens)
HepG2
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using AGO1/EIF2C1 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-AGO1/EIF2C11 antibody (lanes under 'anti-AGO1'). Asterisk indicates heavy chain of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- AGO1_HepG2.png
AGO1 (Homo sapiens)
K562
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using AGO1/EIF2C1 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-AGO1/EIF2C11 antibody (lanes under 'anti-AGO1'). Asterisk indicates heavy chain of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- AGO1_K562.png
AGO1 (Homo sapiens)
compliant
- Caption
- Western blot following shRNA against EIF2C1 in HepG2 whole cell lysate using EIF2C1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2C1. EIF2C1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- EIF2C1-HEPG2-FU'S.png