1. Antibodies
  2. Homo sapiens
  3. ZNF217

ENCAB182PZR

Antibody against Homo sapiens ZNF217

Homo sapiens
MCF-7, K562, GM12878
characterized to standards with exemption
Homo sapiens
HepG2
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-265A
Lot ID
1
Characterized targets
ZNF217 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1029
External resources

Characterizations

ZNF217 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-265A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 115.272.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZNF217 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A303-265A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 115.272
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZNF217 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A303-265A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZNF217 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody A303-265A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 115.272.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
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 5.jpg
ZNF217 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody A303-265A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 115.272.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZNF217 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
exempt from standards
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A303-265A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
CHD4 and HDAC1 (55KD), HDAC2 (55KD) are in one complex, ZNF217 and HDAC1, HDAC2 are components of BHC complex, CHD4 are also part of this complex, http://www.genecards.org/cgi-bin/carddisp.pl?gene=HDAC2&keywords=hdac2 and http://www.genecards.org/cgi-bin/carddisp.pl?gene=HDAC1&keywords=hdac1. HDAC1 and HDAC2 are not in the same gel slice with CHD4 and ZNF217. Both HNRNPUL1 and ZNF217 have interactions with ELAVL1 (36KD), http://thebiogrid.org/113547/summary/homo-sapiens/znf217.html and http://thebiogrid.org/116281/summary/homo-sapiens/hnrnpul1.html. ELAVL1 is not in the same gel slice with HNRNPUL1 and ZNF217. Both ZNF217 and KDM1A are components of histone deacetylase complex, http://www.genecards.org/cgi-bin/carddisp.pl?gene=ZNF217&keywords=znf217.
Reviewer comment
Although chromatin-associated protein CHD4 is ranked higher by enrichment than ZNF217, the submitters note that CHD4 and ZNF217 may be part of the same complex.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford