ENCAB000BCU
Antibody against Homo sapiens RFX1
Homo sapiens
K562, MCF-7
characterized to standards
Homo sapiens
GM12878, HepG2, HeLa-S3, liver
not characterized to standards
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-10650
- Lot ID
- H2108
- Characterized targets
- RFX1 (Homo sapiens)
- Host
- goat
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Epitope mapping within an internal region of RFX1 of human origin
- External resources
Characterizations
RFX1 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-10650. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1057_2_RFX1_sc-10650.jpg
RFX1 (Homo sapiens)
compliant
- Caption
- The motif for target RFX1 is represented by the attached position weight matrix (PWM) derived from ENCFF653BOL. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.963986448 Global enrichment Z-score: 8.713755402 Positional bias Z-score: 12.96617763 Peak rank bias Z-score: 4.140652295 Enrichment rank: 1.0
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
RFX1 (Homo sapiens)
GM12878
not compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody sc-10650. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- The size conflicts with Uniprot, the gel is unreadable
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- expt1065_5-RFX1 (D-19)-sc-10650.jpg
RFX1 (Homo sapiens)
MCF-7
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-10650. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 130.0
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- MCF7-RFX1.jpg
RFX1 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
not compliant
- Caption
- a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3,MCF7, Liver using the antibody sc-10650.
- Reviewer comment
- Multiple bands, none >50 of total signal in lane. Expected size (~130kD) not indicated.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- RFX1_sc-10650_WB_a.jpg
RFX1 (Homo sapiens)
HeLa-S3
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: HelaS3, using the antibody sc-10650 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~104 kDa.
- Reviewer comment
- Band not within 20% of expected size
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- RFX1_sc-10650_WB_b.jpg