1. Antibodies
  2. Homo sapiens
  3. SP1

ENCAB000BAV

Antibody against Homo sapiens SP1

Homo sapiens
K562, HepG2, HeLa-S3, MCF-7, HEK293T
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
GM12878, liver
not characterized to standards
Status
released
Source (vendor)
Sigma
Product ID
S9809
Lot ID
107K4853
Characterized targets
SP1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic Sp1 peptide: Lys-Ser-Met-Pro-Gly-Leu-Gln-Thr- Ile-AsnLeu-Ser-Ala-Leu-Gly-Thr-Ser-Gly-Ile-Gln conjugated to KLH with glutaraldehyde as immunogen.
Antigen sequence
Lys-Ser-Met-Pro-Gly-Leu-Gln-Thr- Ile-AsnLeu-Ser-Ala-Leu-Gly-Thr-Ser-Gly-Ile-Gln
External resources

Characterizations

SP1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody S9809. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 95/106.
Reviewer comment
Immunoreactive signal near 60kD is stronger than that near the expected size but it may also be in the IgG lane... tough call.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SP1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody S9809. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 95/106
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SP1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody S9809. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
High background, multiple bands present and difficult to determine whether signal at indicated size comprises of >50% of the total in the lane.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SP1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody S9809. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Multiple bands but band of expected size is >50% of the total signal in the lane.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SP1 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target SP1 is represented by the attached position weight matrix (PWM) derived from ENCFF191QSX. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.62725018301 Global enrichment Z-score: 4.15225981433 Positional bias Z-score: 6.33375158649 Peak rank bias Z-score: 10.476923864 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
SP1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody S9809. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 95/106
Submitter comment
<50% band intensity
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SP1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T using the antibody S9809. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 95/106.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SP1 (Homo sapiens)
GM12878K562HepG2HeLa-S3liver
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HepG2, HelaS3 and Liver using the antibody S9809. Molecular mass: 80693 Da
Reviewer comment
Band is not 50% of overall signal (Lanes 1, 3, 5). Lanes 2 and 4 are compliant.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
SP1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody S9809 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford