ENCAB000AJV
Alternate accession: ENCAB799JRJ
Antibody against Homo sapiens KAT2B
Homo sapiens
HeLa
partially characterized
Homo sapiens
A549, H1, K562
not characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-666A
- Lot ID
- 1
- Characterized targets
- KAT2B (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- bradley-bernstein:PchAb 154
- External resources
Characterizations
KAT2B (Homo sapiens)
not reviewed
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
- Download
- human_PCAF_validation_Bernstein.pdf
KAT2B (Homo sapiens)
HeLa
compliant
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
- Download
- human_PCAF_validation_Bernstein.pdf
KAT2B (Homo sapiens)
A549H1
not compliant
- Caption
- Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected in a range below the expected size (110kDa).
- Reviewer comment
- Band intensity is not 50% of total signal for both lanes
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
KAT2B (Homo sapiens)
K562K562
not compliant
- Caption
- K562 whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction along side a whole cell lysate were loaded on a CriterionXT gel and separated. After separation, the samples were transferred using a wet transfer. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed. Band of expected size visualized representing strongest signal in the lane.
- Reviewer comment
- No IgG control
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- pcaf_bethyl_A300-666A-1_IP.png