ENCAB000AHK
Antibody against Homo sapiens HDAC6
Homo sapiens
K562, HepG2, GM12878, SK-N-SH
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-341A
- Lot ID
- 1
- Characterized targets
- HDAC6 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- bradley-bernstein:PchAb 290
- External resources
Characterizations
HDAC6 (Homo sapiens)
K562HepG2GM12878SK-N-SH
compliant
- Caption
- Nuclear lysates from K562 (10ug), HepG2 (7ug), GM12878 (7ug), SK-N-SH (6ug), were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a PVDF membrane using wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a band of slightly above the expected size (~131kDa) was detected, probably due to post translational modifications.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
HDAC6 (Homo sapiens)
K562
compliant
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
HDAC6 (Homo sapiens)
exempt from standards
- Submitter comment
- These characterizations were done in ENCODE2, before the current characterization methods.
- Reviewer comment
- These are being exempted as prior to standards. The associated primaries look good.
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570