1. Antibodies
  2. Homo sapiens
  3. ETS1

ENCAB000AGG

Antibody against Homo sapiens ETS1

Homo sapiens
GM12878, GM23338
characterized to standards with exemption
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-350
Lot ID
A1608
Characterized targets
ETS1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG

Characterizations

ETS1 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target ETS1 is represented by the attached position weight matrix (PWM) derived from file ENCFF247QHA. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.912565918559 Global enrichment Z-score: 2.20867828879, Positional bias Z-score: 2.47249556494, Peak rank bias Z-score: 1.69493576998, Enrichment rank: 8.0.
Submitted by
Aditi Narayanan
Lab
Richard Myers, HAIB
Download
 ENCAB000AGG.pdf
ETS1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment from submitter
Attachment from submitters
not reviewed
Caption
ENCODE data standards recognizes various methodologies for secondary validation of antibodies. Among these methodologies is immunoprecipitation followed by mass spectrometry analysis. Briefly, GM12878 whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. Gel was stained with Coomasie Blue in order to visualize marker bands. A gel fragment corresponding to the band indicated above in the western blot image at ~52 kDa was excised and sent to the University of Alabama at Birmingham Cancer Center Mass Spectrometry/Proteomics Shared Facility. There the sample was run on an LTQ XL Linear Ion Trap Mass Spectrometer with alternating collision-induced dissociation and electron-transfer dissociation. Peptides were identified using MASCOT (Matrix Science), with probability based matching at p < 0.05. Subsequent analysis was performed in Scaffold (Proteome Software, Inc.) at 0.0% protein FDR and 1.7% peptide FDR. As per ENCODE data standards, all Scaffold results are listed below, including common contaminants. Target protein is highlighted in bold font. [No match for ETS1 was found by mass spec analysis in the band at ~80 kDa.]
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
ETS1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
ETS1 immunoblot: IP-western with sc-350 ETS1 antibody in whole cell lysate of GM12878. ETS1 band is indicated at ~52 kDa. Expected size is ~50 kDa.
Submitter comment
--
Reviewer comment
The antibody review panel has decided that antibodies that pass ENCODE2 standards, and are only missing their IgG controls for ENCODE3 standards, will be passed via exemption.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
ETS1 (Homo sapiens)
GM23338
Method: immunoblot
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
The ENCODE Binding Working Group finds for some valuable biosamples that recreating a primary on well characterized antibodies is not cost effective. Therefore, they allow exemption from standards for these samples.
Submitter comment
The lab is asking for an exemption forIPSCs due to the lack of resources to make a primary characterization for them
Reviewer comment
Exempted by the Feb 29, 2016 antibody review panel
Submitted by
Richard Myers
Lab
Richard Myers, HAIB