ENCAB000AFI
Alternate accession: ENCAB433VED
Antibody against Homo sapiens CHD7
Homo sapiens
HeLa, K562, GM12878
partially characterized
Homo sapiens
HepG2, endothelial cell of umbilical vein
not characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-223A
- Lot ID
- 1
- Characterized targets
- CHD7 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- bradley-bernstein:PchAb 357, michael-snyder:AS-1570
- External resources
Characterizations
CHD7 (Homo sapiens)
K562
not compliant
- Caption
- K562 whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction along side a whole cell lysate were loaded on a CriterionXT gel and separated. After separation, the samples were transferred using a wet transfer. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed. Band of expected size visualized representing strongest signal in the lane.We assume that since one of the isoforms is relatively big, it goes through degradation and therefore we get more bands in unexpected areas.
- Reviewer comment
- No IgG
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- CHD7_Bethyl_A301-223A-1_IP.png
CHD7 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
compliant
- Caption
- Nuclear lysates from K562 (10ug), HepG2 (10ug), GM12878 (7ug), HUVEC (10ug), were loaded into a 3-8% Tris-acetate gel in 1X TA running buffer. After separation, the samples were transferred to a PVDF membrane using wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Multiple bands can be detected at expected sizes (~360kDa,130kDa and 100kDa). We assume that since one of the isoforms is relatively big, it goes through degradation and therefore we get more bands in unexpected areas.
- Submitter comment
- 335 kDa according to genecards
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- CHD7_Bethyl_A301-223A-1_MH.png
CHD7 (Homo sapiens)
not reviewed
- Caption
- Antibody was validated by use of correlation analysis of ChIP-String data obtained using this antibody together with diverse other antibodies
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
CHD7 (Homo sapiens)
HeLa
compliant
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570